FIG. 4.

Timing of the FoxO1a-to-cGKIα pathway in differentiating C2C12 cells. (A) Western blot analysis of the indicated proteins in differentiating C2C12 cells over a 5-day time course. Uniform protein loading and homogeneous transfer of sodium dodecyl sulfate-polyacrylamide gel electrophoresis membranes were confirmed by staining with FastGreen. (B) Graphic representation of the induction of cGKIα, P-VASP (Ser²³⁹), and myosin heavy chain (Myo HC) protein expression in C2C12 cells, normalized for FastGreen staining.