FIG. 11.

Effect of high expression of hLaΔ316-332 on luciferase activity. U87 cells were transiently cotransfected with the luciferase reporter containing the 3′ UTR of PAM (see construct b in Fig. 10 A) and increasing amounts of either hLa-wt or hLaΔ316-332 as shown. After 48 h the cells were lysed, and the results are presented as ratios of firefly luciferase activity and sea pansy (Renilla) luciferase activity, used as a control. Data from three experiments (n = 3 for each plasmid concentration in three experiments) were used to calculate the mean luciferase activity. Data are presented as mean ± SEM. The asterisks indicate that the values are significantly different (P < 0.001).