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. 2005 Aug;25(15):6485–6495. doi: 10.1128/MCB.25.15.6485-6495.2005

FIG. 1.

FIG. 1.

The UNC-5 cytosolic domain interacts with SRC-1 in vitro. (A) SRC-1 binds the UNC-5 cytosolic domain. HEK293 cells were transfected with the indicated expression vectors. SRC-1-myc was immunoprecipitated (IP) with anti-myc antibody (9E10) and subjected to Western blot analysis (WB) with the indicated antibodies. (B) The SH2 domain of SRC-1 is important for its interaction with UNC-5. Lysates were subjected to immunoprecipitation with an anti-myc antibody (9E10) and probed for the indicated Western blot analysis. SH2M and SH3M denote SRC-1 mutations in the SH2 domain (R165A) and SH3 domain (W108R W109R), respectively. WT, wild type. (C) PP2 inhibits the interaction between SRC-1 and UNC-5. Transfected HEK293 cells were treated with PP2, an inhibitor of Src family kinases, for 30 min at the indicated concentrations (μM). Lysates were immunoprecipitated with anti-myc antibody (9E10) and probed for the indicated Western blot analysis.