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. 2005 Aug;25(15):6682–6693. doi: 10.1128/MCB.25.15.6682-6693.2005

FIG. 5.

FIG. 5.

Effects of gata-1 gene expressions for phenotype induced by the injection of zMelk-like gene MO and regulation of gata-1 promoter by a Melk-like gene. (A) Lateral views (anterior to left) of embryos injected with either control MO, gata-1 RNA or zMelk-like gene MO are shown. Coinjection of zMelk-like gene MO and either EGFP RNA, or gata-1 RNA was done and views of these embryos at 24 and 48 hpf and 5 days (lateral view with anterior to left) are shown. Scale bars, 100 μm. (B) Effects of zMelk-like gene for activation of zebra fish gata-1 promoter in the embryos. Zebra fish gata-1 promoter EGFP and mRNA for either control, zMelk-like gene, or zMlke-likeT169A were coinjected into fertilized eggs and the expression of EGFP was examined under fluorescence dissection microscope. Dorsal views of embryos at 14 hpf and lateral views with anterior to left embryos at 18 hpf are shown.