TABLE 3.
Zebra fish gata-1 promoter-dependent EGFP expression in the presence of zMelk-like gene or gata-1
| Material(s) injected | No. of embryos examineda | No. of EGFP-positive embryos (%)
|
||||
|---|---|---|---|---|---|---|
| No expression | Strong level | Weak level | Ectopic | Lethal | ||
| gata-1 promoter EGFP(50 pg) | 65 | 3 (4%) | 5 (7%) | 53 (83%) | 2 (3%) | 2 (3%) |
| gata-1 promoter EGFP(100 pg) + zMelk-like gene (300 pg) | 72 | 4 (6%) | 33 (45%) | 28 (39%) | 3 (4%) | 4 (6%) |
| gata-1 promoter EGFP(100 pg) + gata-1 (200 pg) | 56 | 5 (9%) | 33 (60%) | 7 (12%) | 36 (65%) | 8 (14%) |
a
Expression levels were arbitrary examined under a fluorescence dissection microscope. Typical examples of strong and weak levels are shown in Fig. 5B.