FIG. 5.

Metabolism is not altered in the Myo-Cre/Flox-MCIP1 mice. (A) Western blot analysis of 20 μg of protein from wild-type (WT) and Tg animals. (B) Western blot analysis of PGC-1α protein in 20 μg of protein from WT S (soleus), E (EDL), T (TA), G (gastrocnemius), H (heart), and P (plantaris). (C) RT-PCR of PGC-1α and skeletal α-actin of the collateral legs from panel A and a comparison of relative mtDNA copy number using semiquantitative PCR of the mtDNA gene for cytochrome B (Cyt B) and the endogenous MCIP1 nuclear gene (nucDNA). (D) Citrate synthase, (E) lactate dehydrogenase, and (F) β-hydroxyacyl-CoA dehydrogenase activities in the soleus and EDL muscles of WT and Tg mice.