TABLE 1.
Normal fertility, testis size, and protein content in UBC4 wild-type and knockout micea
| Parameter | Mean ± SEM (n)
|
|
|---|---|---|
| UBC4 (+/+) | UBC4 (−/−) | |
| Litter size (no. of pups) | 7.7 ± 0.6 (9) | 8.1 ± 0.9 (11) |
| Testis wt (mg) | 102 ± 2 (10) | 96 ± 2 (11) |
| Testis protein (mg/testis) | 9.3 ± 1 (5) | 10.8 ± 0.4 (4) |
| Soluble testis protein (mg/testis) | 2.60 ± 0.24 (7) | 2.95 ± 0.12 (8) |
a
Wild-type or knockout male mice were mated with wild-type females, and the sizes of the litters were determined. Testis weights and soluble testis proteins (measured in supernatants of the extracts centrifuged at 100,000 × g) were determined for 15-week-old mice. Total testis proteins were determined by homogenizing testes from 11-week-old mice and determining the protein content in the crude extracts. n, number of animals studied.