FIG. 10.

Time course of MPP⁺ and monoamine uptake in HeLa and 293 T cells expressing IRIP. 293T cells were transfected with hOCT3 (A) or mOAT1 (B) transporters in the presence of either vector pEF/myc-hIRIP or pEF/myc-mIRIP. To evaluate the effect of IRIP overexpression on the uptake activity of neuronal monoamine transporters, HeLa-2 (H2) and HeLa-4 (H4) cells were transfected with hSERT (C), hNET (D), or hDAT (E). Transport activity was measured after adding labeled substrates for different time periods. For all curves, means ± standard errors are presented. Three to four independent experiments are presented in each graph. *, P < 0.05 in comparison to cell lines transfected with transporter and vector plasmid.