FIG. 6.

Induction of H-ferritin by TNF is absent in L929 cells, and ectopically introducing TNF-inducible H-ferritin inhibits TNF-induced LIP, ROS, and cell death. (a) L929 and A549 cells were treated with TNF (100 ng/ml) for different periods of time. H-ferritin mRNA levels were measured by real-time PCR. (b) LIP levels of the cells treated were as described for panel a. (c) ROS production in the cells treated were as described for panel a. (d) Stable lines of L929 cells transfected with H-ferritin in a TNF-inducible expression vector (TI H-ferritin) or empty vector (Vector) were treated with TNF for different periods of time. H-ferritin mRNA levels were examined by semiquantitative PCR. (e) LIP levels in TI H-ferritin- and vector-transfected L929 cells after TNF treatment of different periods of time. wt, wild type. (f) ROS levels in the cells were as described for panel e. (g) Viability of the cells was as described for panel e.