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. 2025 Feb 27;16:1512740. doi: 10.3389/fphar.2025.1512740

FIGURE 7.

FIGURE 7

Compound 4c alleviated the symptoms of acute lung injury in mice. (A), Hematoxylin–eosin (HE) staining of lung tissue sections (magnification, ×100). ALI was established by nasal infusion of LPS. BALB/c mice were intraperitoneally injected with oridonin and 4c. (B), Immunohistochemistry (IHC) staining of lung tissue sections with LCA (CD45) antibody and hematoxylin (magnification, ×300). (C), Quantitation of the inflammatory cells (LCA positive staining cells) in the lung tissue sections. (D‒E), RT‒qPCR assays of lung tissues. (F), WB analysis of lung tissues. (G‒I), Quantification of the WB results for NLRP3, p-NF-κB and IL-6. All the results are expressed as the average value ± SD of three independent experiments. # indicates that the difference between the LPS and control groups is significant; and * indicates that the difference between the oridonin derivative and LPS groups is significant. Student’s t-test is used to calculate the significance, ####and ****, p < 0.0001; ### and ***, p < 0.001; ## and **, p < 0.01; # and *, p < 0.05.