FIG. 10.

Sustained expression of c-Kit, Akt, Rac1, or Rac2 in G1E-ER4 cells does not affect differentiation. (A) G1E-ER4 cells expressing the above-mentioned cDNAs were induced to differentiate in the presence of β-estradiol for 48 h and subjected to flow cytometric analysis to detect the expression of Ter119. Closed histograms indicate staining using an isotype control antibody. Open histograms indicate staining using an antibody against Ter119. (B and C) Cell lysates from panel A were subjected to Western blot analysis using an anti-globin antibody.