FIG. 6.

Chromatin immunoprecipitation demonstrates binding of hnRNP K to the eIF4E promoter. Cell lysates from formaldehyde-treated TGR cells in the indicated growth conditions were immunoprecipitated with nonimmune rabbit serum (lane 2) or with anti-hnRNP K antibody that was coincubated with (lane 4) or without (lane 3) the peptide used to generate the antibody in rabbits (99). DNA was purified from the eluted complex and used as a template in PCRs with primers to a nontranscribed rat locus (AC120715), the rat c-myc promoter (c-myc), the eIF4E promoter (eIF4E), and the 5S rRNA region (5S). PCR products were separated by agarose gel electrophoresis and visualized with ethidium bromide. Input DNA was used as a positive control in lane 1.