FIG. 3.

The CnAβ promoter responds to NFATc4 and GATA4. (A) Transient transfection assays in cultured neonatal cardiac myocytes with the pGL3-CnAβ2322 or pGL3-CnAβ1399 promoter-luciferase fusion constructs and constructs encoding NFATc4 or ΔCnA. Cells were harvested 24 h later, and luciferase activities were measured as relative light units per microgram of protein (*, P < 0.05 versus no cotransfection). (B) Essentially the same experiment shown in panel A, except that 10T1/2 fibroblasts were used. (C) Transient transfection assay in cultured 10T1/2 cells with the pGL3-CnAβ2322 promoter-luciferase fusion construct and a construct encoding GATA4 (*, P < 0.05 versus no cotransfection). Similar results were observed in a total of three independent experiments, each performed in duplicate.