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. 2005 Aug;25(15):6649–6659. doi: 10.1128/MCB.25.15.6649-6659.2005

FIG. 5.

FIG. 5.

Characterization of NFAT and GATA binding elements in the CnAβ proximal promoter. (A and B) The left-hand panels show EMSA reactions between in vitro-translated NFATc4-RHD and the indicated NFAT sites from the CnAβ proximal promoter. The right-hand panels show the same putative NFAT sites reacted with unprogrammed lysates as a control. The arrows show the position of the NFAT-specific mobility shift. The well-characterized NFAT site from the IL-4 promoter was used as a migration control. (C and D) The left-hand panels show EMSA reactions between in vitro-translated GATA4 and the indicated GATA sites from the CnAβ proximal promoter. The right-hand panels show the same putative GATA sites reacted with unprogrammed lysates as a control. The arrows show the position of the GATA4-specific mobility shift. Similar results were obtained with three additional independent experiments.