FIG. 8.
Crossing CnAβ2322-LacZ transgenic mice with ΔCnA and NFATc4 transgenic mice. CnAβ2322-LacZ line 5.2 transgenic mice (A and B) or line 6.2 transgenic mice (C and D) were crossed with cardiac tissue-specific transgenic mice expressing either activated CnA or activated NFATc4. Hearts were removed at 4 weeks of age, and LacZ activity was measured and normalized to total protein concentrations (*, P < 0.05 versus CnAβ2322-LacZ line 5.2; **, P < 0.05 versus CnAβ2322-LacZ line 6.2) (line 5.2, three mice; line 5.2/ΔCnA, three mice; line 6.2, three mice; line 6.2/ΔCnA, three mice; line 5.2/NFATc4, four mice; line 6.2/NFATc4, three mice). (E) HW/BW ratios were significantly increased in double-transgenic mice compared to NTG or CnAβ2322-LacZ transgenic mice (*, P < 0.05 versus NTG or CnAβ2322 single transgenic mice).