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NRG1 is required for 3×NRE25- but not 3×NRE22D-mediated repression; RIM101, DFG16, YGR122w, and VPS36 are required for 3×NR22D- but not 3×NRE25-mediated repression. Colonies of the BY4741-derived deletion strains, as indicated on the right side of the panel, that contained pLGn+NRE42, pLGn+3×NRE25, or pLGn+3×NRE22D, as indicated at the top of the panel, were overlaid with X-Gal-containing agar. WT, wild type.
