Expression of the CYC1-NRE42-lacZ reporter gene and the (−537)DIT1-lacZ reporter gene is higher in an nrg1 rim101 strain than in either an nrg1 strain or a rim101 strain. (A) Colonies of wild-type (WT) (W303-B), nrg1Δ (KRY302), rim101Δ (KRY308), and nrg1Δ rim101Δ (KRY318) cells and tup1Δ (Y169) cells containing pLG312n, a plasmid with a CYC1-lacZ reporter gene (no insert); pLGn+NRE42, a plasmid with a CYC1-NRE42-lacZ reporter gene; and p(−537)DIT1-lacZ, a plasmid with a (−537)DIT1-lacZ reporter gene, were overlaid with X-Gal-containing agar. (B) The table gives units of β-galactosidase activity measured in extracts from cells containing pLG312 (no insert) or pLGn+NRE42 (NRE42).