Figure 1.

Cloning of 2 novel Dido transcripts. (A) Dido transcript expression was analyzed in murine (left) and human (right) cell lines by hybridization with a probe corresponding to full-length Dido1. Blots were stained with methylene blue to normalize the amounts loaded. (B) Schematic representation of Dido1, Dido2, and Dido3. Different colors indicate common and specific nucleotide sequences. (C and D) Flag-tagged Dido1, Dido2, and Dido3 expression was analyzed in immunoblot (C) or immunofluorescence (D) of transfected 293T cells using anti-Flag M2 mAb. Molecular size markers are indicated at left (C). Magnification, ×100.