Fig. 3. 2-DE analysis of a R. etli protein profile stimulated by biotin.

Total cellular proteins were prepared from a MM-biotin culture, isolated and analyzed by 2-DE protein electrophoresis. (A) Complete 2-D protein profile of R. etli grown in MM-biotin; (B) enlarged regions of the protein profile from biotin-stimulon; (C) enlarged region of the protein profile from fermentative metabolism (MM-without biotin). The boxed area in (A) shows the area of the gels enlarged for (B) and (C). Arrows indicate the proteins induced only with biotin.