Figure 2.

Morphology of corneal surface in normal and T1D animals. (A) Corneal basal cells were stained with hematoxylin and eosin and photographed using an Olympus BX50 microscope at an original magnification of ×40. Scale bar, 25 µm. (B) Histograms representing the number of basal corneal epithelial cells per 200 µm (mean ± SEM). (C) Representative images of Ki67⁺ staining of corneal epithelium after 15 days of NTX treatment. Scale bar, 50 µm. (D) Histograms represent the number per 500 µm (mean ± SEM) of Ki67⁺ cells in corneal epithelium. (E) Corneal tissue stained with anti-OGFr. Scale bar, 50 µm. (F) Histogram represents optical density for OGFr staining (mean ± SEM) in rats after 15 days of NTX treatment. Data were analyzed using a two-factor ANOVA (condition, sex), with the post hoc Tukey's multiple comparisons. Significant differences were noted as *P < 0.05; **P < 0.01; ***P < 0.001; or ****P < 0.0001.