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Effect of ETFQO on cellular energy metabolism. A. RNA interference efficiency in ETFQO expression was assessed by Western blot. B. The Seahorse XF real-time ATP ratio assay was performed to quantify ATP production from OXPHOS and glycolysis. C.The extracellular acidification rate (ECAR) was measured using the Seahorse XF Glycolysis Stress Test to evaluate the effects on glycolysis. *P < 0.05, **P < 0.01, and ****P < 0.001 by 2-tailed, Two-way ANOVA test. Data represent the mean ± SD
