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. 2025 Mar 12;20:121. doi: 10.1186/s13023-025-03640-4

Fig. 4.

Fig. 4

RNA-seq analysis of HEK293 cell lines with ETFDH gene knockdown. A. A total 184 differentially expressed genes were detected in si-ETFDH group compared with control group. B. Gene ontology (GO) terms. C. Heatmap of selected differentially expressed genes. D. Real-time PCR validation of differential expression of HSPA1B, ACSM3, ZNF267 and ETFDH Genes. E. Oil Red O staining to assess lipid accumulation in HEK293T cells after ETFDH gene knockdown and PA stimulation. For all statistical tests: *, P < 0.05; ***, P < 0.001