Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1988 Sep;403:27–39. doi: 10.1113/jphysiol.1988.sp017236

Circulatory effects of a depilatory dose of mouse epidermal growth factor in sheep.

N B Carter 1, A A Fawcett 1, J R Hales 1, G P Moore 1, B A Panaretto 1
PMCID: PMC1190700  PMID: 3267020

Abstract

1. Haemodynamic parameters and tissue blood flow rates were measured in two groups of five sheep infused I.V. for 24 h with either saline or 128.6 micrograms mouse epidermal growth factor (mEGF) kg-1 body weight. Measurements were made preinfusion and at +3, +12, +24, +27 and +48 h. We wished to assess relationships between blood flow rates and known functional changes in various organs during EGF treatment, especially any relationship between skin blood flow rate and the known depilatory effects of the protein in sheep. 2. Cardiac output increased and total peripheral resistance and mean arterial pressure decreased during and after infusion in the mEGF-treated group relative to the control group. 3. The greatest increase in blood flow rates occurred in woolled skin (+500%) during mEGF infusion, a result which in itself may have been disparate with the known depilatory effects of EGF. The mucosas of the alimentary tract (except abomasum) and the submaxillary and sublingual salivary glands also showed vasodilatation. 4. There were short-term increases in pituitary and adrenal gland blood flow that may have been associated with the corticotrophin-releasing factor properties of EGF. Flow in the thyroids showed the greatest increase post-infusion when thyroid hormone metabolism may have been reverting to normal. Blood flow rates decreased in the pancreas and perirenal fat. 5. Our general conclusion was that mEGF had specific vasodilator effects in the skin, the thyroid, submaxillary and sublingual glands and the mucosas of most of the alimentary tract.

Full text

PDF
28

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell A. W., Hales J. R., King R. B., Fawcett A. A. Influence of heat stress on exercise-induced changes in regional blood flow in sheep. J Appl Physiol Respir Environ Exerc Physiol. 1983 Dec;55(6):1916–1923. doi: 10.1152/jappl.1983.55.6.1916. [DOI] [PubMed] [Google Scholar]
  2. Berk B. C., Brock T. A., Webb R. C., Taubman M. B., Atkinson W. J., Gimbrone M. A., Jr, Alexander R. W. Epidermal growth factor, a vascular smooth muscle mitogen, induces rat aortic contraction. J Clin Invest. 1985 Mar;75(3):1083–1086. doi: 10.1172/JCI111772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bower J. M., Camble R., Gregory H., Gerring E. L., Willshire I. R. The inhibition of gastric acid secretion by epidermal growth factor. Experientia. 1975 Jul 15;31(7):825–826. doi: 10.1007/BF01938488. [DOI] [PubMed] [Google Scholar]
  4. Cantin M., Genest J. The heart and the atrial natriuretic factor. Endocr Rev. 1985 Spring;6(2):107–127. doi: 10.1210/edrv-6-2-107. [DOI] [PubMed] [Google Scholar]
  5. Chabot J. G., Payet N., Hugon J. S. Effects of epidermal growth factor (EGF) on adult mouse small intestine in vivo and in organ culture. Comp Biochem Physiol A Comp Physiol. 1983;74(2):247–252. doi: 10.1016/0300-9629(83)90595-9. [DOI] [PubMed] [Google Scholar]
  6. Corcoran J. M., Waters M. J., Eastman C. J., Jorgensen G. Epidermal growth factor: effect on circulating thyroid hormone levels in sheep. Endocrinology. 1986 Jul;119(1):214–217. doi: 10.1210/endo-119-1-214. [DOI] [PubMed] [Google Scholar]
  7. Elder J. B., Ganguli P. C., Gillespie I. E., Gerring E. L., Gregory H. Effect of urogastrone on gastric secretion and plasma gastrin levels in normal subjects. Gut. 1975 Nov;16(11):887–893. doi: 10.1136/gut.16.11.887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gan B. S., MacCannell K. L., Hollenberg M. D. Epidermal growth factor-urogastrone causes vasodilatation in the anesthetized dog. J Clin Invest. 1987 Jul;80(1):199–206. doi: 10.1172/JCI113048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gerring E. L., Gregory H. Candidate hormones of the gut. 8. Urogastrone. Gastroenterology. 1974 Oct;67(4):739–740. [PubMed] [Google Scholar]
  10. Green M. R., Basketter D. A., Couchman J. R., Rees D. A. Distribution and number of epidermal growth factor receptors in skin is related to epithelial cell growth. Dev Biol. 1983 Dec;100(2):506–512. doi: 10.1016/0012-1606(83)90243-9. [DOI] [PubMed] [Google Scholar]
  11. Hales J. R., Cliff W. J. Direct observations of the behaviour of microspheres in microvasculature. Bibl Anat. 1977;(15 Pt 1):87–91. [PubMed] [Google Scholar]
  12. Hales J. R., Fawcett A. A., Bennett J. W. Radioactive microsphere partitioning of blood flow between capillaries and arteriovenous anastomoses in skin of conscious sheep. Pflugers Arch. 1978 Aug 25;376(1):87–91. doi: 10.1007/BF00585252. [DOI] [PubMed] [Google Scholar]
  13. Hales J. R., Schleger A. V., Kemp D. H., Fawcett A. A. Cutaneous hyperaemia elicited by larvae of the cattle tick, Boophilus microplus. Aust J Biol Sci. 1981;34(1):37–46. [PubMed] [Google Scholar]
  14. Hollis D. E., Chapman R. E., Panaretto B. A., Moore G. P. Morphological changes in the skin and wool fibres of Merino sheep infused with mouse epidermal growth factor. Aust J Biol Sci. 1983;36(4):419–434. doi: 10.1071/bi9830419. [DOI] [PubMed] [Google Scholar]
  15. LEVI-MONTALCINI R., COHEN S. Effects of the extract of the mouse submaxillary salivary glands on the sympathetic system of mammals. Ann N Y Acad Sci. 1960 Mar 29;85:324–341. doi: 10.1111/j.1749-6632.1960.tb49963.x. [DOI] [PubMed] [Google Scholar]
  16. Moore G. P., Panaretto B. A., Wallace A. L. Treatment of ewes at different stages of pregnancy with epidermal growth factor: effects on wool growth and plasma concentrations of growth hormone, prolactin, placental lactogen and thyroxine and on foetal development. Acta Endocrinol (Copenh) 1984 Apr;105(4):558–566. doi: 10.1530/acta.0.1050558. [DOI] [PubMed] [Google Scholar]
  17. Muramatsu I., Hollenberg M. D., Lederis K. Vascular actions of epidermal growth factor-urogastrone: possible relationship to prostaglandin production. Can J Physiol Pharmacol. 1985 Aug;63(8):994–999. doi: 10.1139/y85-164. [DOI] [PubMed] [Google Scholar]
  18. Nadel E. R., Fortney S. M., Wenger C. B. Effect of hydration state of circulatory and thermal regulations. J Appl Physiol Respir Environ Exerc Physiol. 1980 Oct;49(4):715–721. doi: 10.1152/jappl.1980.49.4.715. [DOI] [PubMed] [Google Scholar]
  19. Panaretto B. A., Moore G. P., Robertson D. M. Plasma concentrations and urinary excretion of mouse epidermal growth factor associated with the inhibition of food consumption and of wool growth in Merino wethers. J Endocrinol. 1982 Aug;94(2):191–202. doi: 10.1677/joe.0.0940191. [DOI] [PubMed] [Google Scholar]
  20. Polk D. H., Ervin M. G., Padbury J. F., Lam R. W., Reviczky A. L., Fisher D. A. Epidermal growth factor acts as a corticotropin-releasing factor in chronically catheterized fetal lambs. J Clin Invest. 1987 Mar;79(3):984–988. doi: 10.1172/JCI112910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Savage C. R., Jr, Cohen S. Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization. J Biol Chem. 1972 Dec 10;247(23):7609–7611. [PubMed] [Google Scholar]
  22. Takayanagi I. Effects of urogastrone on mechanical activities of the stomach and intestine of guinea-pig. J Pharm Pharmacol. 1980 Mar;32(3):228–230. doi: 10.1111/j.2042-7158.1980.tb12901.x. [DOI] [PubMed] [Google Scholar]
  23. Vale W., Rivier C., Brown M. R., Spiess J., Koob G., Swanson L., Bilezikjian L., Bloom F., Rivier J. Chemical and biological characterization of corticotropin releasing factor. Recent Prog Horm Res. 1983;39:245–270. doi: 10.1016/b978-0-12-571139-5.50010-0. [DOI] [PubMed] [Google Scholar]
  24. Zerbe G. O., Walker S. H. A randomization test for comparison of groups of growth curves with different polynomial design matrices. Biometrics. 1977 Dec;33(4):653–657. [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES