Table 2.

Thermal profiling (PCR conditions) of PhaR

Steps	Temperature	Time	Cycles
Initial denaturation	94 °C	1 min	X1
Final denaturation	98 °C	10 s	X32
Annealing	55 °C	15 s
Initial extension	68 °C	15 s sec
Final extension	68 °C	3 min	X1
Hold	12 °C	∞	X1