Table 1.
The upgraded technology for isolation and purification of the enzyme L-lysine-α-oxidase from Trichoderma harzianum Rifai
| Stage of enzyme isolation | Fraction volume, ml | Total protein, mg | Total activity, U | Specific activity U/mg of protein | Yield in activity, % | Purification degree |
|---|---|---|---|---|---|---|
| Precipitation (NH4)2SO4 | ||||||
| 0 | 300 | 420 | 4200 | 10 | 100 | 0 |
| 15% (NH4)2SO4 | 200 | 348 | 4060 | 11 | 96,7 | 1,1 |
| 70% (NH4)2SO4 | 30 | 195 | 4000 | 20,5 | 95,2 | 2,1 |
| Chromatography | ||||||
| DEAE-Sefacel | 140 | 0,20 | 3800 | 135 | 90.4 | 13,5 |
| Sephadex G-100 | 80 | 0,14 | 3300 | 292 | 78,6 | 29,2 |