Table 2. Physicochemical and reactogenic profiles of NB in controls and optimization series.

Metric	NB22	18a3	7e12	v0	v1	v2	v3	v4	v5
pI (>8)	8.41	8.78	8.07	9.56	9.64	9.64	9.85	9.79	9.64
Instability (<40)	38.95	42.06	45.69	35.2	38.19	42.34	47.51	41.62	38.34
Solubility (>0.45)	0.569	0.578	0.560	0.614	0.659	0.678	0.684	0.684	0.665
Melting temperature (>55°C)	68.4	65.2	64.8	66.4	70.3	69.1	67.5	68.6	69.5
Toxicity (<0.6)	0.33	0.35	0.28	0.55	0.53	0.54	0.55	0.54	0.53
Allergenicity (AlgPredv2) (<0.3)	0.39	0.9	0.78	-0.21	-0.20	-0.18	0.32	0.32	0.32
Allergenicity (AllerTopv2) (PN)	PA	PN	PA	PN	PN	PN	PN	PN	PN
Antigenicity (bacteria, virus, tumor) (<0.4, <0.4, <0.5)	1.00, 0.52, 0.67	1.07, 0.63, 0.66	1.07, 0.67, 0.68	0.59, 0.43, 0.47	0.57, 0.42, 0.48	0.49, 0.40, 0.48	0.56, 0.45, 0.45	0.66, 0.44, 0.46	0.67, 0.44, 0.50