表 1. Primer design.

引物设计

Gene name	Primer sequences (5' to 3')	Product length/bp
GAPDH: glyceraldehyde 3-phosphate dehydrogenase; IL-1β: interleukin-1 beta; IL-18: interleukin-18; Caspase-1: cysteine-aspartic proteases; NLRP3: NLR family pyrin domain containing 3; TNF-α: tumor necrosis factor alpha; TLR4: Toll-like receptor 4; I-κB: inhibitor of κB.
GAPDH	F: CAGCCTTCCTTCTTGGGTAT	106
	R: CTGTGTTGGCATAGAGGTCTT
IL-1β	F: TGTGTTTTCCTCCTTGCCTCTGAT	105
	R: TGCTGCCTAATGTCCCCTTGAAT
IL-18	F: GCTGTGACCCTCTCTGTGAA	158
	R: TCCATCTTGTTGTGTCCTGGA
Caspase-1	F: TGTCAGGGGCTCACTTTTCA	164
	R: AACGTTTTGTCAGGGTCACC
NLRP3	F: GTCTGGAAGAACAGGCAACAT	144
	R: AGAACTGTCATAGGGTCAAAACG
TNF-α	F: AAGTCAACCTCCTCTCTGCC	191
	R: TCCAAAGTAGACCTGCCCG
TLR4	F: GCCCTACCAAGTCTCAGCTA	165
	R: CTGCAGCTCTTCTAGACCCA
I-κB	F: GAGCCGACCTCAATAAACCG	324
	R: TGCGACTGTGAACCACGATG