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. 2025 Mar 4;16:1551625. doi: 10.3389/fmicb.2025.1551625

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Copyright © 2025 Liu, Geng, Ye, Xu, Zheng, Wang, Lei, Wu, Jiang, Hu, Chen, Yan, Guo and Qiu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of binding relationships between ChsD and aap-milR10516-x and between mkh1 and aap-milR-2478-y. (A) Peak diagram of Sanger sequencing of the amplified binding sites. (B) Dual-luciferase reporter gene assay of the binding relationship between aap-milR10516-x and ChsD. (C) RT-qPCR detection of aap-milR10516-x and ChsD. (D) Peak diagram of Sanger sequencing of the mutated binding sites. (E) Dual-luciferase reporter gene assay binding relationship between aap-milR-2478-y and mkh1. (F) RT-qPCR detection of aap-milR-2478-y and mkh1. The dual-luciferase assay data were presented as mean ± standard deviation (SD) and analyzed by two-sided Student’s t-test; ns, p > 0.05; **p < 0.01; ***p < 0.001.