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Isolation, culture, and identification of ASCs. (A) ASCs were extracted from the antler mesenchymal layer. (B) Primary and passaged culture of ASCs. (C) IHC staining showed positive expression of vimentin and negative expression of keratin in ASCs. (D) Flow cytometry detected positive expression of ASCs surface antigens CD29, CD44, CD90, and CD105 and negative expression of CD31 and CD45.
