Figure 4.

The role of TLR4 in the H/R injury and effect of propofol preconditioning on the protein and mRNA expression of TLR4. (A) Cell viability was detected in H/R, E, P and E + P groups under hypoxia 1.5 hours/reoxygenation 2 hours. Each value represents the mean ± standard deviation (n = 6). a p < 0.01, ## p < 0.01 vs. H/R group. b p < 0.05 vs. E group. c p < 0.05 vs. P group. (B) The protein expression of TLR4 was determined by Western blotting analyses. Each value represents the mean ± standard deviation (n = 6). **p < 0.01 vs. N group. ## p < 0.01 vs. H/R group. (C) The mRNA expression of TLR4 was determined by quantitative real‐time polymerase chain reaction. Each value represents the mean ± standard deviation (n = 3). **p < 0.01 vs. N group. ## p < 0.01 vs. H/R group.