Fig. 7.

ML329 improved skin fibrosis in vivo. (A) The keloid-bearing mouse model construction and subcutaneous ML329 injection. (B) Grafts were collected on day 21 and separated into saline and ML329 groups. (C-D) Volume and weight measurement of grafts. (E) HE and Masson stain the collected grafts. Scale bar, 200 μm. (F) Sircol assay showed a decrease in ECM in the ML329 groups. (G-I) Measurements of melanin, total iron, Fe²⁺, Fe³⁺ levels, and MDA levels in KD grafts. (J-M) Immunofluorescence assay of FTH1⁺S100A4⁺ fibroblast cells and GPX4⁺S100A4⁺ fibroblast cells. Scale bar, 200 μm. N = 6. *P < 0.05; **P < 0.001; ***P < 0.001