Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1988 Aug;402:533–541. doi: 10.1113/jphysiol.1988.sp017219

Effect of ionic composition of milk on transepithelial potential in the goat mammary gland.

D R Blatchford 1, M Peaker 1
PMCID: PMC1191906  PMID: 2466984

Abstract

1. A method is described for altering the ionic composition of the alveolar (residual) milk in the goat mammary gland. Transepithelial (blood-milk) potential difference (PD) was recorded before and after composition was changed. 2. When [Na+] and [K+] were varied while [Na+ + K+] and [Cl-] were maintained constant, there were no significant changes in PD. 3. When [Na+] was varied, with [K+] and [Cl-] constant, there was a linear relation between PD and log [Na+], with a change of 18 mV per tenfold change in concentration. 4. When [K+] was varied, with [Na+] and [Cl-] constant, there was a linear relation between PD and log [K+], with a change of 24 mV per tenfold change. 5. In view of the lack of discrimination between Na+ and K+ in the first experiment, the sum of [Na+] and [K+] was considered. In the experiments in which [Na+] or [K+] were varied, there was a change of 37 and 38 mV, respectively, per tenfold change in [Na+ + K+]. The quantitative effects on PD of changing [Na+] or [K+] when expressed as changes in [Na+ + K+] were not significantly different. 6. When [Cl-] was varied, with [Na+] and [K+] constant, there was a linear relation between PD and log [Cl-], with a change of 22 mV per tenfold change. 7. The results are discussed in relation to the types of ion channel that might be present in the apical membrane of the secretory cell and in relation to hypotheses on the mechanisms of ion transport in the mammary gland.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
533

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biber T. U., Drewnowska K., Baumgarten C. M., Fisher R. S. Intracellular Cl activity changes of frog skin. Am J Physiol. 1985 Sep;249(3 Pt 2):F432–F438. doi: 10.1152/ajprenal.1985.249.3.F432. [DOI] [PubMed] [Google Scholar]
  2. Evans M. H., Linzell J. L., Peaker M. Membrane potentials in the mammary gland of the lactating rat. J Physiol. 1971 Mar;213(2):49P–50P. [PubMed] [Google Scholar]
  3. Holt C. Swelling of Golgi vesicles in mammary secretory cells and its relation to the yield and quantitative composition of milk. J Theor Biol. 1983 Mar 21;101(2):247–261. doi: 10.1016/0022-5193(83)90339-9. [DOI] [PubMed] [Google Scholar]
  4. LINZELL J. L. Mammary-gland blood flow and oxygen, glucose and volatile fatty acid uptake in the conscious goat. J Physiol. 1960 Oct;153:492–509. doi: 10.1113/jphysiol.1960.sp006550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Linzell J. L., Peaker M. Changes in colostrum composition and in the permeability of the mammary epithelium at about the time of parturition in the goat. J Physiol. 1974 Nov;243(1):129–151. doi: 10.1113/jphysiol.1974.sp010746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Linzell J. L., Peaker M. Intracellular concentrations of sodium, potassium and chloride in the lactating mammary gland and their relation to the secretory mechanism. J Physiol. 1971 Aug;216(3):683–700. doi: 10.1113/jphysiol.1971.sp009547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Linzell J. L., Peaker M. The effects of oxytocin and milk removal on milk secretion in the goat. J Physiol. 1971 Aug;216(3):717–734. doi: 10.1113/jphysiol.1971.sp009549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Linzell J. L., Peaker M. The permeability of mammary ducts. J Physiol. 1971 Aug;216(3):701–716. doi: 10.1113/jphysiol.1971.sp009548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Peaker M., Blatchford D. R. Distribution of milk in the goat mammary gland and its relation to the rate and control of milk secretion. J Dairy Res. 1988 Feb;55(1):41–48. doi: 10.1017/s0022029900025838. [DOI] [PubMed] [Google Scholar]
  10. Peaker M. Mechanism of milk secretion: milk composition in relation to potential difference across the mammary epithelium. J Physiol. 1977 Sep;270(2):489–505. doi: 10.1113/jphysiol.1977.sp011964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Peaker M., Taylor J. C. Milk secretion in the rabbit: changes during lactation and the mechanism of ion transport. J Physiol. 1975 Dec;253(2):527–545. doi: 10.1113/jphysiol.1975.sp011205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shen S. S., Hamamoto S. T., Bern H. A., Steinhardt R. A. Alteration of sodium transport in mouse mammary epithelium associated with neoplastic transformation. Cancer Res. 1978 May;38(5):1356–1361. [PubMed] [Google Scholar]
  13. Siemen D., Reuhl T. Non-selective cationic channel in primary cultured cells of brown adipose tissue. Pflugers Arch. 1987 May;408(5):534–536. doi: 10.1007/BF00585082. [DOI] [PubMed] [Google Scholar]
  14. Van Driessche W., Zeiske W. Ionic channels in epithelial cell membranes. Physiol Rev. 1985 Oct;65(4):833–903. doi: 10.1152/physrev.1985.65.4.833. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES