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. 2025 Mar 20;21:188. doi: 10.1186/s12917-025-04627-w

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Micrographs show protozoan parasites isolated from fly pools stained with different stains. Cryptosporidium spp. oocysts, a = Direct smear, b = Iodine stained, and c, d = Ziehl–Nielsen stained, 100 × oil immersion. Giardia spp. cyst, e = Direct smear, f = Iodine stained, and g = Methylene blue stained 40x. Entamoeba spp. cyst, h = Direct smear, i = Iodine stained 40x. Balantidium coli trophozoites, j = iodine-stained, k = methylene blue-stained 40x. l = Trichomonas spp. trophozoites stained with methylene blue, 40x