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. 2005 Jul 29;5:24. doi: 10.1186/1475-2867-5-24

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Copyright © 2005 Chandar et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunohistochemical staining of beta-catenin and p53. Cells were treated with 10^-11M 17-beta estradiol for 48 h as described under methods and stained for p53 (green) and beta-catenin (red) using specific antibodies. Panel A shows cells strongly staining for both proteins in the nucleus (broken arrow), strong p53 staining in the nucleus and beta-catenin at the plasma membrane (solid arrows) and strong staining of p53 in the nucleus with beta-catenin relegated to the cytoplasm (arrow head). B and C show apoptotic cells with strong staining of both proteins.