Table 2.
List of gRNA Oligos for in Vitro and in Vivo Validation
| Target gene | gRNA | Sense | In vitro oligo sequence | In vivo oligo sequence |
|---|---|---|---|---|
| Non-targeting | sg-NT | Fwd | caccgGCTCCTGGGGCAGTCACCCA | accGCTCCTGGGGCAGTCACCCA |
| Rev | aaacTGGGTGACTGCCCCAGGAGCc | aacTGGGTGACTGCCCCAGGAGC | ||
| Vps72 | sg1-Vps72 | Fwd | caccgGAACCCTGGTACTGTGCCCT | accGAACCCTGGTACTGTGCCCT |
| Rev | aaacAGGGCACAGTACCAGGGTTCc | aacAGGGCACAGTACCAGGGTTC | ||
| sg2-Vps72 | Fwd | caccgGCACTCATAGGCAGCGCCTA | accGCACTCATAGGCAGCGCCTA | |
| Rev | aaacTAGGCGCTGCCTATGAGTGCc | aacTAGGCGCTGCCTATGAGTGC | ||
| sg3-Vps72 | Fwd | caccgGCGCCTAGGGCACAGTACCA | accGCGCCTAGGGCACAGTACCA | |
| Rev | aaacTGGTACTGTGCCCTAGGCGCc | aacTGGTACTGTGCCCTAGGCGC | ||
| sg4-Vps72 | Fwd | caccgGGGCTGCTGTGGCCACTCAT | accGGGCTGCTGTGGCCACTCAT | |
| Rev | aaacATGAGTGGCCACAGCAGCCCc | aacATGAGTGGCCACAGCAGCCC | ||
| sg5-Vps72 | Fwd | caccgGTTCACCTTAAGTCTCTTTG | accGTTCACCTTAAGTCTCTTTG | |
| Rev | aaacCAAAGAGACTTAAGGTGAACc | aacCAAAGAGACTTAAGGTGAAC | ||
| Zbtb7b | sg1-Zbtb7b | Fwd | caccgGACAGTGTCCGCAGTGGCCA | accGACAGTGTCCGCAGTGGCCA |
| Rev | aaacTGGCCACTGCGGACACTGTCc | aacTGGCCACTGCGGACACTGTC | ||
| sg2-Zbtb7b | Fwd | caccgGAGGAGGCAAAGGGTGGCGG | accGAGGAGGCAAAGGGTGGCGG | |
| Rev | aaacCCGCCACCCTTTGCCTCCTCc | aacCCGCCACCCTTTGCCTCCTC | ||
| sg3-Zbtb7b | Fwd | caccgGAGGGCTGGACGGGGGTCGG | accGAGGGCTGGACGGGGGTCGG | |
| Rev | aaacCCGACCCCCGTCCAGCCCTCc | aacCCGACCCCCGTCCAGCCCTC | ||
| sg4-Zbtb7b | Fwd | caccgGTGACAGAGGGTGTTGCTAA | accGTGACAGAGGGTGTTGCTAA | |
| Rev | aaacTTAGCAACACCCTCTGTCACc | aacTTAGCAACACCCTCTGTCAC | ||
| sg5-Zbtb7b | Fwd | caccgGTGGAAGCCAGGTGAATGTA | accGTGGAAGCCAGGTGAATGTA | |
| Rev | aaacTACATTCACCTGGCTTCCACc | aacTACATTCACCTGGCTTCCAC | ||
| Gba | sg1-Gba | Fwd | caccgGGAATTTTGGGGAGGCCTAA | accGGAATTTTGGGGAGGCCTAA |
| Rev | aaacTTAGGCCTCCCCAAAATTCCc | aacTTAGGCCTCCCCAAAATTCC | ||
| sg2-Gba | Fwd | caccgGGGCCTCCCCAAAATTCACA | accGGGCCTCCCCAAAATTCACA | |
| Rev | aaacTGTGAATTTTGGGGAGGCCCc | aacTGTGAATTTTGGGGAGGCCC | ||
| sg3-Gba | Fwd | caccgGTAAGCCGGTCACGTGATGC | accGTAAGCCGGTCACGTGATGC | |
| Rev | aaacGCATCACGTGACCGGCTTACc | aacGCATCACGTGACCGGCTTAC | ||
| sg4-Gba | Fwd | caccgGTGAATACAGTGGCCCTCAC | accGTGAATACAGTGGCCCTCAC | |
| Rev | aaacGTGAGGGCCACTGTATTCACc | aacGTGAGGGCCACTGTATTCAC | ||
| sg5-Gba | Fwd | caccgGTGTATGGACAGCTGAGTAG | accGTGTATGGACAGCTGAGTAG | |
| Rev | aaacCTACTCAGCTGTCCATACACc | aacCTACTCAGCTGTCCATACAC | ||
| Mrpl9 | sg1-Mrpl9 | Fwd | caccgGCTCTCCGCTGTCCTATCGT | accGCTCTCCGCTGTCCTATCGT |
| Rev | aaacACGATAGGACAGCGGAGAGCc | aacACGATAGGACAGCGGAGAGC | ||
| sg2-Mrpl9 | Fwd | caccgGGCCTGAGTGGTATAGATAT | accGGCCTGAGTGGTATAGATAT | |
| Rev | aaacATATCTATACCACTCAGGCCc | aacATATCTATACCACTCAGGC | ||
| sg3-Mrpl9 | Fwd | caccgGGGCGTGCAAGGCGGGGTTG | accGGGCGTGCAAGGCGGGGTTG | |
| Rev | aaacCAACCCCGCCTTGCACGCCCc | aacCAACCCCGCCTTGCACGCCC | ||
| sg4-Mrpl9 | Fwd | caccgGTGCCCGCGGCGCGCCTGAG | accGTGCCCGCGGCGCGCCTGAG | |
| Rev | aaacCTCAGGCGCGCCGCGGGCACc | aacCTCAGGCGCGCCGCGGGCAC | ||
| sg5-Mrpl9 | Fwd | caccgGTTAAAAAAAGAGGCGGAGG | accGTTAAAAAAAGAGGCGGAGG | |
| Rev | aaacCCTCCGCCTCTTTTTTTAACc | aacCCTCCGCCTCTTTTTTTAAC |