Figure 2.

Auditory dysfunction and cochlear sensory cell loss post-cyclodextrin treatment. (A) ABRs were measured in both cyclodextrin-treated (six weeks after cyclodextrin treatment) and control mice. In the treated mice, ABR thresholds are significantly elevated across all tested frequencies, with higher frequency responses (24 kHz and 32 kHz) becoming undetectable. n=the number of mice. (B) Cochleogram shows complete OHC loss in the basal region and partial loss in the middle and apical regions. (C) Comparison of the damage levels across the three rows of OHCs. The shaded areas marked the cochlear regions where three rows of OHC display different levels of damage, with the first row showing significantly greater loss. OHC1, OHC2, and OHC3 represent the first, second, and third rows of OHCs, respectively. n=the number of cochleae. (D) Comparison of the level of IHC loss among different cochlear regions from the apex to the base. IHC loss is most prominent in the basal region, with surviving IHCs in the middle and apical regions. Each dot represents one viewing area, and the data were collected from 6 cochleae.