Figure 4.

The structural integrity of Deiters’ cells in regions with severe OHC loss. (A) A 3D view of the organ of Corti stained for actin and IFIT3. (B and C) A confocal image created by digital sectioning of the organ of Corti to highlight Deiters’ cells. (D) Actin staining showing complete OHC loss in the middle region of a cyclodextrin-treated cochlea. (E) IFIT3 staining of the same region reveals relatively intact central stalks and phalangeal processes of Deiters’ cells. (F) Merged view of the images from (D) and (E). (G) Actin staining in a cochlear region with both missing and surviving OHCs. Thin lines highlight the contours of a missing (left) and a surviving OHC (right). (H and I) IFIT3 staining reveals that Deiters’ cells beneath both surviving and missing OHC regions show similar structures in their central stalks and phalangeal processes. The lines in panel H outline the contours of the central stalks of the two Deiters’ cells.