TABLE 1.
Percentage of inhibition in transport observed in cross-competition assaya
| Competitor | % of inhibition of l-Ser uptake | % of inhibition of l-Thr uptake | % of inhibition of Gly uptake |
|---|---|---|---|
| l-Ser | –b | 82.7 ± 1.0 | 83.2 ± 1.4 |
| d-Ser | 39.65 ± 1.02 | 22.5 ± 2.3 | 31 ± 3.6 |
| Gly | 45.01 ± 5.50 | 42.3 ± 3.1 | – |
| l-Thr | 50 ± 3.48 | – | 58 ± 2.8 |
| l-Ala | 21.90 ± 4.02 | – | – |
| d-Ala | 25.12 ± 1.38 | – | – |
| l-Cys | 30.88 ± 7.24 | – | – |
| l-His | No inhibition | – | – |
| l-Leu | No inhibition | – | – |
| l-Phe | No inhibition | – | – |
a
Cross-competition assay between l-Ser, l-Thr, and Gly: transport inhibition of each amino acid was determined by adding an excess (10 × KM value) for each amino acid tested. All data were shown as mean ± SD (n = 3). All experiments were replicated three times or more in three biological replicates.
b
–, not done.