FIG. 3.

Protein synthesis in cell-free assays. Krebs-2 lysates programmed with mengovirus RNA were labeled with [³⁵S]methionine as described in Materials and Methods. The proteins were fractionated by polyacrylamide gel electrophoresis and detected by autoradiography. Reactions contained no RNA (lane 1); no additions (lane 2); 80 μM dipyridamole (DPM, lane 3); or 1.25% ethanol (EtOh, lanes 3 and 4). The migration positions of known mengovirus proteins are indicated.