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. 2005 Sep;79(17):10915–10922. doi: 10.1128/JVI.79.17.10915-10922.2005

FIG. 2.

FIG. 2.

Immunoblot analysis of mutant LEF-3 expression in transfected versus infected cells. (A) Sf21 cells were transfected with pHSEHLEF3-C (lane 1), pHSHLEF3-N (lane 2), pHSEHLEF3 (lane 3), or pIE-1hrlef3 (lane 4) or infected with AcMNPV (lane 5, multiplicity of infection of 1, 24 h postinfection). The transfected cells were heat shocked at 20 h posttransfection at 42°C for 30 min and returned to 28°C for 3.5 h before harvesting. Extracts were analyzed by SDS-PAGE and immunoblotting using a polyclonal antibody against LEF-3. Mock-transfected cell extracts are also shown (lane 6). (B) Sf21 cells were transfected in duplicate with pHSEHLEF3 (lane 2 and 3), pHSEHLEF3-C (lane 4 and 5) or pHSHLEF3-N (lane 6 and 7). One set of cells was heat shocked (lanes 3, 5, and 7) while the others were not (lanes 2, 4, and 6). At 24 h posttransfection, extracts were prepared, and immunoblotted and probed as described for panel A. Cells infected with AcMNPV (lane 1, multiplicity of infection of 1, 24 h postinfection) or mock transfected (lane 8) are also shown.