FIG. 4.

Schematic diagram of GFP fusion constructs. Plasmids were constructed that expressed GFP (represented by the stippled areas), LEF3 amino acids 2 to 56 (represented by the white areas) fused with GFP [LEF-3 (2-56)-GFP], GFP fused with P143 (represented by the gray areas) (GFP-P143), or LEF3 amino acids 5 to 56 fused with GFP and P143 [LEF-3 (5-56)-GFP-P143]. Each protein was fused at the N terminus with the same 22-amino-acid sequence including the 6XHis tag (His, black areas). The length of each area is drawn to the same scale according to the number of amino acids contained in each region of the fusion proteins.