FIG. 6.

Intracellular localization of GFP-P143 in the presence of LEF-3. Sf21 cells were transfected with pHSEHGFP-P143, or cotransfected with plasmids pHSEHGFP-P143 plus pHSEHLEF3, pHSEHLEF3-N, pHSEHLEF3(2-125), pHSHLEF3-C, or pHSEHLEF3(2-83/126-385). The cells were harvested at 24 h posttransfection, fixed, and either observed directly for GFP fluorescence or probed with polyclonal anti-AcMNPV LEF-3 (1:1,000) antibody. Immunofluorescence was detected with secondary goat anti-rabbit immunoglobulin G conjugated with Alexa Fluor 568. Cell nuclei were detected by DAPI staining. Ninety to 100% of the positively transfected cells showed the patterns represented in each panel.