TABLE 1.
Primer | Name | Sequence | Product length (bp) |
---|---|---|---|
A | ΔgB KanrF | CTT TTT CGG TTC AAC CTG GAG CAG ACG TGC CCA GAC ACC AAA GAC AAG TAC CAC CAA GAA GGA ATT TTA CTG TAT CTG GAC AAG GGA AAA CGC AAG C | 1,953 |
B | ΔgB KanrR | AAT AAA ATT TAT GAA TCC GGT AAC CAA TGA GCC ACA TAG AGT GAC CAC GCT GCT GGC CAC GTT CAC CAC CGT TAA GGA GAA AAT ACC GCA TCA GGA A | |
C | ΔgB int F | ACT TCC ACA CAA CAG GCG GAC TCT A | 808 |
D | ΔgB int R | GTC TCC AGG TCA AAC ACG CTA CTC G | |
E | ΔgB flank F | TCC AGT TCC CCC ACA CCC CCA GGA T | 2,284 |
F | ΔgB flank R | GCA TTC CCA GCA GGA TGT TTT TGA T |