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. 2005 Sep;79(17):11412–11421. doi: 10.1128/JVI.79.17.11412-11421.2005

TABLE 4.

In vitro and in vivo characteristics of the reverse-genetic viruses

Virus Glycosylation Cleavage site sequence ECEa
PFU/ml in CEF cells
Intravenous pathotyping in chickens
EID50/ml MDTb Trypsin No trypsin Morbidityc Mortalityd Virus titere Hi titerf
Parent NST (+) PQ——RKKR 9.7 >5 8 × 109 none 1/10 0/0 5.8 ± 0.4 8.2 ± 0.6
rg1 NST (+) PQ——RKKR 8.9 >5 1 × 109 none 0/10 0/10 1.5 ± 2.5 4.7 ± 1.4
rg2 NSK (−) PQ——RKKR 9.2 >5 3 × 108 none NDg ND ND ND
rg3 KST (−) PQ——RKKR 10.5 >5 4 × 108 none 0/10 0/0 <1.0 2.1 ± 1.8
rg4 NST (+) PQ—RRKKR 9.2 3.6 2 × 109 none 0/10 0/0 <1.0 5.2 ± 1.0
rg5 NST (+) PQRKRKKR 10.9 2.2 4 × 107 1 × 106 10/10 1/10 3.3 ± 1.4 6.8 ± 0.7
rg6-CK/Scot/59 H5 KST (−) PQ——RKKR 10.5 2.2 3 × 107 3 × 105 10/10 3/10 2.5 ± 0.1 6.0 ± 1.3
a

The challenge doses were adjusted to 104 EID50/0.2 ml and 106 EID50/0.2 ml for the pathogenicity test in ECE and chickens, respectively.

b

MDT, average number of days required to kill the inoculated eggs.

c

Number of birds that manifested clinical signs/number of birds inoculated.

d

Number of birds that died/number of birds inoculated.

e

The mean virus titer of three samples is expressed as the log10 EID50 per milliliter ± standard deviation.

f

Log2 hemagglutination inhibition titer (mean titer of sera collected from the surviving birds 10 days after challenge) ± standard deviation.

g

ND, test not done.