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Cleavage pattern of CHCV169* with endoproteinase Glu-C. CHCV169* was treated with endoproteinase Glu-C as described in Materials and Methods and subjected to a 15% polyacrylamide Tricine gel electrophoresis (61). Lane 1, control CHCV169*. Lanes 2 to 7 show CHCV169*, digested during increasing lengths of time: lane 2, 5 min; lane 3, 15 min; lane 4, 30 min; lane 5, 1 h; lane 6, 2 h; and lane 7, 3 h.
