TABLE 1.
Sequences inserted at the 3′ end of the minigenome Le region
| Minigenome | Sequence of 3′-terminal extensiona |
|---|---|
| +4 | UGCCb |
| +5 | UGCCA |
| +6 | UGCCAA |
| +7 | UGCCAAU |
| +8 | UGCCAAUA |
| +9 | UGCCAAUAG |
| +14 | UGCCAAUAGUGUUU |
| +25 | UGCCUUAGAUAACUCAAUAGUGUUU |
| +56 | UGCCUAGGUCGUUGUUUGUUAGCUCGGUCUUCUCUUGAUGGUUCCAAAUACUUAGG |
| +56(11) | UGCCCUUUUUUCCUAGGGUUAGCUCGGUCUUCUCUUGAUGGUUCCAAAUACUUAGG |
| +56(13) | UGCCCUUUUUUACCCUAGGUAGCUCGGUCUUCUCUUGAUGGUUCCAAAUACUUAGG |
| +56(13)Nsic | UGCCCUUUUUUACCCUAGGUAGCUCGGUCUUCUCUUGAUGGUUCCAAAUACgUAGG |
| 3Aextd | UGACCUUUUUUACCCUAGGUAGCUCGGUCUUCUCUUGAUGGUUCCAAAUACgUAGG |
| +13 only | UGCCCUUUUUUACCCUAGGUAGCUCGGUCUUCUCUUGAUGGUUCCAAAUACUUAGG |
| +56(35) | UGCCCUUUUUUACGCAUGUUGUUUGAACGUAUUUGCCUAGGUUCCAAAUACUUAGG |
| +56(44) | UGCCCUUUUUUACGCAUGUUGUUUGAACGUAUUUGGUUUUUUUACCUAGGCUUAGG |
| Wild typee | UGCCCUUUUUUACGCAUGUUGUUUGAACGUAUUUGGUUUUUUUACCCCGUUUAUUC |
a
The sequence is shown 3′ to 5′ as negative-sense RNA.
b
Nucleotides that share identity with the corresponding position in wild-type Le are shown in bold type.
c
The U-to-G substitution to introduce an NsiI site in the plasmid cDNA is in lowercase.
d
The C-to-A substitution at position 3 is underlined.
e
The sequence at the 3′ terminus of wild-type RSV is shown for comparison, with the Le sequence in bold type and the gene start sequence in italics.