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Role of specific IE proteins and UL13 in causing the loss of Ser-2 phosphorylation on the CTD. Vero (A) or HEL (B) cells were mock infected (lanes 1), infected with WT HSV-1 (lane 2), or infected with the virus mutants indicated (lanes 3 to 8). Protein extracts were prepared at 9 hpi and analyzed by immunoblotting with MAb H5 to detect Ser-2-phosphorylated LS. Cellular protein EEA1 was also analyzed.
