TABLE 3.
Retrovirus-specific primers used for amplification of pet chip hybridization probes
| No. | Nucleotide sequencea | Target of amplification (accession no.)b |
|---|---|---|
| Forward primers | ||
| 1 | GGAGAATAGGTTCTTCCTCAGAAG | Canine ERV-L (AJ233669) |
| 2 | GGAGAATAGAGACTACCACAGGGG | VL30 (AF053745) |
| 3 | GGAGAATAGGTTTTGCCTCGAGGA | Rat ERV-L (AJ233604) |
| 4 | GGAGAATAGGTTTTGCCCCAGGGT | MMTV (M15122) |
| 5 | GGAGAATAGGTTTTACCACAAGG | EAV-HP (AJ292966); MPMV (M12349); FeSFV (U78765) |
| 6 | GGAGAATAGGTTTACCACAAGGA | EiAV (M87581); FIV2 (U56928) |
| 7 | GGAGAATAGGTCTTACCACAGGGA | IAP-2 (U58494) |
| 8 | GGAGAATAGGTTCTACCACAAGGA | BIV (M32690) |
| 9 | GGAGAATAGGTTCTCCCBCAGGG | MusD1 (AF246632); JSRV (A27950); BFV (AY134750) |
| 10 | GGAGAATAGTATCTCCCACAGGGC | MusD2 (AF246633) |
| 11 | GGAGAATAGGTTCTTTCTTAAGAC | MMTV-like mRE (AL606472) |
| 12 | GGAGAATAGGTTCTACCCCAACTC | mRE (XM_141084) |
| 13 | GGAGAATAGGTTCTGCCTCGGGGA | mRE (XM_146956) |
| 14 | GGAGAATAGTTTCTACCTCAAGGA | mRE (XM_143828) |
| 15 | GGAGAATAGGTTTTGCCTCAAGTA | MuERVL (Y12713) |
| 16 | GGAGAATAGAGCTTACCACAGGG | FIV (M59418); RLV (M77194); MiEV (X99931) |
| 17 | GGAGAATAGAGACTCCCACAGGG | MLV (J02255); VL30-like mRE (AL844168) |
| 18 | GGAGAATAGGTCCTACCTCAGGG | IAP-1 (X87638); feline ERV-L (AJ233664); BLV (K02120); bovine ERV-L (AJ233662) |
| 19 | GGAGAATAGCGGCTACCACAAGGG | GaLV (M26927) |
| 20 | GGAGAATAGAGACTTCCACAAGGA | mRE (XM_136338) |
| 21 | GGAGAATAGCAACTTCCACAAGGA | OvEV (X99932) |
| 22 | GGAGAATAGCGCTTCCCACAAGGA | BoEV (X99924) |
| 23 | GGAGAATAGCGCCTTCCHCAAGGG | RVwp (AJ236133); FeLV (L06140); BaEV (D10032) |
| 24 | GGAGAATAGGTCTTGCCCCAAGGG | ALV (Z46390) |
| 25 | GGAGAATAGCGACTGCCCCAAGGG | PERV (AF038600) |
| 26 | GGAGAATAGAGGTCGCGACAGAGG | MuRVY (X87639) |
| 27 | GGAGAATAGTGGTAACCCCAGGAG | MuRRS (X02487) |
| 28 | GGAGAATAGGTTCTGCTGGAGGTT | mRE (AL513352) |
| 29 | GGAGAATAGGTCTGTAGTCAGATG | mRE (AL672245) |
| Reverse primers | ||
| 30 | GGAGAACATCAAGACATCATCTGTGAA | MusD1/2 (AF246632/3) |
| 31 | GGAGAAGAAAAGGATATCGTCCATATA | BLV (K02120) |
| 32 | GGAGAAATATATATATCATCCACATA | JSRV (A27950); FIV (M59418); FIV2 (U56928); FeSFV (U78765) |
| 33 | GGAGAAAAGAGGATGTCATCCATGTA | MMTV (M15122/AL606472); IAP-2 (U58494); can. ERV-L (AJ233669); MPMV (M12349) |
| 34 | GGAGAAAATCAAAATGTCATCCATATA | IAP-1 (X87638) |
| 35 | GGAGAACAGCATAATGTCATCAATATA | MuERV-L (Y12713) |
| 36 | GGAGAATAGCAAAAGATCATCCATATA | EAV-HP (AJ292966); ALV (Z46390) |
| 37 | GGAGAAAATCAACAAATCATCCATATA | BIV (M32690) |
| 38 | GGAGAACACGAACAAATCATCCATATA | EiAV (M87581) |
| 39 | GGAGAACAGAAGCAGGTCATCCACGTA | PERV (AF038600) |
| 40 | GGAGAATAGAAGAAGGTCATCAACAAA | RLV (M77194) |
| 41 | GGAGAACAGCAGTAAGTCATCYACGTA | MLV (J02255) |
| 42 | GGAGAACAGCAGTARGTCATCTACATA | FeLV (M18247) |
| 43 | GGAGAACAAGAGGAGGTCATCTACATA | BaEV (D10032) |
| 44 | GGAGAACACATAGACGTCATCCACATA | BFV (AY134750) |
| 45 | GGAGAACAGTAGTATGTCATCCACGTA | OvEV (X99932); MiEV (X99931) |
| 46 | GGAGAAACCAAGAGGTCGTCCACATA | BoEV (X99924); GaLV (M26927); VL30-like mRE (AL844168) |
| 47 | GGAGAAGATTAAAAGATCATCTACGTA | VL30 (AF053745); bovine ERV-L (AJ233662) |
| 48 | GGAGAAAAGGATTAAATCATCTCCATA | MuRVY (X87639) |
| 49 | GGAGAAAAGGAGCAAGTCATCAACATA | MuRRS (X02487) |
| 50 | GGAGAACAAAAGAAGGTCATCTATATA | mRE (XM_136338) |
| 51 | GGAGAACAATAGGAGATCATCTACATA | mRE (AC127359.4) |
| 52 | GGAGAAACAGAAGACATCATCATCATA | mRE (XM_146956) |
| 53 | GGAGAAGAGAAGGACATCATCATCATA | mRE (XM_143828) |
| 54 | GGAGAAGAGAAGAATATCATCTATATA | mRE (XM_141084) |
| 55 | GGAGAAAATCAAAATATCATCCATACT | mRE (AL513352) |
| 56 | GGAGAAATAAAAAATATCACCCATACT | mRE (AL672245) |
| 57 | GGAGAACATAATGTCATCCAGGTA | Bovine ERV-L (AJ233662) |
| 58 | GGAGAAGTCATCCAGGTAATCCAG | Bovine ERV-L (AJ233662) |
| 59 | GGAGAATAGCAACAAATACCATTG | RVwp (AJ236133) |