FIG. 1.

(A) Schematic view of the wild-type AAV-2 genome and of the wild-type p5 element. (B) Mapping of the Rep68 nicking sites on the p5 element. Nicking reactions were performed on a p5 substrate (nucleotides 190 to 353 of wild-type AAV-2) labeled on either strand in the presence of 1 pmol of Rep68 as described in the Materials and Methods section. The reaction products were resolved on 6% denaturing polyacrylamide gels along with a sequencing reaction to precisely map the nicking sites. The five identified nicking sites are indicated, as well as the position of the outstanding p5 elements.