TABLE 1.

Description of the p5 mutant versions used in in vivo and in vitro replication assays

p5 version	Length (bp)	Positionsa	TATA box	Rep binding site	trs+1	Hairpinb
p5wt	163	190-353	+	+	+	+
p5mtrs	163	190-353	+	+	−	−
p5mRBS	162	190-353	+	−	+	+
p5D3	116	237-353	+	+	+	+
p5D5	73	237-310	+	+	+	+
p5D7	72	233-304	+	+	+	+
p5D10	55	250-304	+	+	+	+
p5D10mTATA	56	249-304	−	+	+	+
p5D12	45	260-304	−	+	+	+
p5D14	44	250-293	+	+	+	−
p5D6	33	261-293	−	+	+	−

^aOn the AAV-2 sequence (GenBank accession no. NC 001401).

^bPutative hairpin present at the trs⁺¹ (Fig. 2B).